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Analysis

1.WO/2020/181947METHOD FOR DETECTING ONE OR MORE TARGET NUCLEIC ACID SEQUENCES TO BE TESTED BY SINGLE TUBE AND KIT THEREOF
WO 17.09.2020
Int.Class C12Q 1/6851
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
6851Quantitative amplification
Appl.No PCT/CN2020/074925 Applicant JIANGSU MACRO&MICRO-TEST MED-TECH CO., LTD. Inventor LIU, Licheng
Provided are a method for detecting one or more target nucleic acid sequences to be tested by a single tube and a kit thereof. The method comprises the steps of: designing specific isothermal amplification primers and fluorescent probe RNHPs for each target nucleic acid sequence to be tested, and labeling the fluorescent probe RNHPs for each target nucleic acid sequence to be tested with different fluorescences; in the presence of a ribonuclease RNaseH, isothermally amplifying each target nucleic acid sequence to be tested under the action of a nucleic acid polymerase; and forming hybridization chain products labeled with different fluorescences by means of different target nucleic acid sequences to be tested, thereby achieving multiplex detection of a plurality of target nucleic acid sequences to be tested. In the present invention, on the basis of an isothermal amplification technique, real-time multiplex isothermal detection can be achieved just by introducing a special probe RNHP and combining same with RNaseH2.
2.WO/2020/177397RESPIRATORY SYNCYTIAL VIRUS UNIVERSAL RAPID DETECTION PRIMER SET AND KIT
WO 10.09.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/CN2019/120067 Applicant SUZHOU CLICKGENE BIOTECH CO., LTD. Inventor GAO, Anguo
The invention provides a respiratory syncytial virus (RSV) universal rapid detection primer set and a kit. The respiratory syncytial virus universal rapid detection primer set comprises upstream and downstream primers and probes capable of simultaneously detecting an F gene sequence of respiratory syncytial virus type A and type B. The primer set comprises 1 upstream primer, 2 downstream primers, and 1 probe.
3.20200281930HUMAN PAPILLOMA VIRUS AS PREDICTOR OF CANCER PROGNOSIS
US 10.09.2020
Int.Class A61K 31/519
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
33Heterocyclic compounds
395having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
495having six-membered rings with two nitrogen atoms as the only ring hetero atoms, e.g. piperazine
505Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
519ortho- or peri-condensed with heterocyclic rings
Appl.No 16654582 Applicant FOUNDATION MEDICINE, INC. Inventor Chris Hendrik Boshoff

Methods of treating a head and neck cancer are disclosed.

4.20200277678Biomarkers For Head And Neck Cancer And Methods Of Their Use
US 03.09.2020
Int.Class C12Q 1/6886
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
Appl.No 16857767 Applicant Shi-long LU Inventor Shi-long LU

Disclosed is a diagnostic panel of methylated genomic loci encoding microRNA (mgmiR) markers that demonstrated 90% sensitivity and 100% specificity in the detection of head and neck squamous cell carcinoma (HNSCC). These results represent the first use of quantitative MS-PCR for the detection of mgmiRs. In addition, this panel demonstrates the ability to detect hypermethylation in the adjacent mucosa of cancer patients, suggesting its utility in early detection. This panel is also capable of detecting cancer by using saliva, blood and FNA tissue samples.

5.20200277679METHODS AND COMPOSITIONS FOR TARGETED SINGLE-STRANDED CLEAVAGE AND TARGETED INTEGRATION
US 03.09.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 16875152 Applicant Sangamo Therapeutics, Inc. Inventor Jianbin Wang

Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.

6.WO/2020/173121DOUBLE PCR DETECTION METHOD FOR SUGARCANE WHITE LEAF DISEASE PHYTOPLASMA AND XANTHOMONAS ALBILINEANS AND PRIMER SET THEREOF
WO 03.09.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No PCT/CN2019/115465 Applicant SUGARCANE RESEARCH INSTITUTE, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES Inventor ZHANG, Rong-Yue
Disclosed are a double PCR detection method for sugarcane white leaf disease phytoplasma and Xanthomonas albilineans and a primer set thereof. The present invention designs a pair of specific primers for the tuf gene of sugarcane white leaf disease phytoplasma and the rpoD gene of Xanthomonas albilineans, respectively, and by means of optimization, establishes a double PCR reaction system that is capable of simultaneously detecting the sugarcane white leaf disease phytoplasma and the Xanthomonas albilineans.
7.1020200102251마이크로플루이딕칩 기반 낭충봉아부패병 현장진단용 프라이머 세트, 이를 이용한 낭충봉아부패병을 진단하는 방법 및 키트
KR 31.08.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 1020190020657 Applicant 주식회사 진시스템 Inventor 조윤상
본 발명은 낭충봉아부패병 현장진단용 프라이머 세트에 관한 것으로서, 서열번호 1의 정방향 프라이머 및 서열번호 2의 역방향 프라이머로 이루어진 프라이머 쌍, 서열번호 3의 정방향 프라이머 및 서열번호 2의 역방향 프라이머로 이루어진 프라이머 쌍, 서열번호 4의 정방향 프라이머 및 서열번호 2의 역방향 프라이머로 이루어진 프라이머 쌍, 서열번호 1의 정방향 프라이머 및 서열번호 6의 역방향 프라이머로 이루어진 프라이머 쌍, 서열번호 7의 정방향 프라이머 및 서열번호 2의 역방향 프라이머로 이루어진 프라이머 쌍 또는 서열번호 8의 정방향 프라이머 및 서열번호 2의 역방향 프라이머로 이루어진 프라이버 쌍으로 이루어진 군에서 하나 이상을 포함한다. 상기와 같은 본 발명에 따르면, 현장에서 낭충봉아부패병을 신속하고 효과적으로 진단하고, 확직하는데 우수한 효과가 있을뿐만 아니라, 그에 소모되는 시간과 비용 절감이 가능하며, 다양한 시료의 병성감정에 유용하게 이용할 수 있는 효과가 있다.
8.111593146基于RNA荧光原位杂交的高灵敏度单分子RNA病毒检测方法
CN 28.08.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 202010564232.5 Applicant 马彬 Inventor 马彬
本发明涉及一种基于RNA荧光原位杂交的高灵敏度单分子RNA病毒检测方法,包括以下步骤:a)处理样本和制作细胞学和病毒涂片;b)使病毒RNA与地高辛或者生物素标记的探针结合;c)通过抗体结合标记的探针;d)用荧光或者过氧化酶底物显色;e)用显微镜检测涂片,鉴定病毒RNA。通过本发明的检测方法的准确率可以达到90%以上,例如新型冠状病毒(SARS‑CoV‑2)。
9.111593142一种同时检测包括SARS-CoV-2的九种呼吸道病毒的检测试剂盒
CN 28.08.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 202010469523.6 Applicant 山东艾克韦生物技术有限公司 Inventor 王海岩
本发明公开了一种同时检测包括SARS‑CoV‑2的九种呼吸道病毒的检测试剂盒,属于生物检测技术领域。本发明的试剂盒包括甲型流感病毒、乙型流感病毒、新型冠状病毒、鼻病毒、副流感病毒、呼吸道合胞病毒、冠状病毒229E亚型、冠状病毒OC43亚型、冠状病毒HKU1亚型的引物组和荧光探针,以及反应液、酶混合液、阳性质控品、阴性质控品。本发明可实现包括新型冠状病毒SARS‑CoV‑2在内的九种常见呼吸道病毒的快速确认与筛查,同时本发明使用实时荧光定量PCR技术,试剂盒具备灵敏度高,特异性好。
10.111593138一种鸭乙型肝炎病毒重组聚合酶等温扩增检测方法
CN 28.08.2020
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 201910894805.8 Applicant 山东省农业科学院家禽研究所 Inventor 马秀丽
本发明公开了一种鸭乙型肝炎病毒重组聚合酶等温扩增检测方法,以及专用的RPA引物对(如SEQ ID NO.1和SEQ ID NO.2所示)、试剂盒。本发明根据鸭乙型肝炎病毒的基因序列设计特异性引物,进一步优化建立了用于快速准确进行鸭乙型肝炎病毒检测的重组酶聚合酶等温扩增方法。该方法可特异性地检测出鸭乙型肝炎病毒,其最低检出模板量为146pg,灵敏度与传统PCR相当。由于整个基因扩增只需一个温度,不需要特殊的仪器设备,操作更简单、快速,适用于生产中鸭乙型肝炎病毒的现场快速检测。