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1. WO2020228218 - HIRUDIN AND APPLICATION THEREOF

Publication Number WO/2020/228218
Publication Date 19.11.2020
International Application No. PCT/CN2019/107585
International Filing Date 24.09.2019
IPC
C07K 7/08 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
7Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04Linear peptides containing only normal peptide links
08having 12 to 20 amino acids
C12N 15/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
12Genes encoding animal proteins
A61K 38/10 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
10Peptides having 12 to 20 amino acids
A61P 9/10 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
10for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
A61P 9/10
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
10for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
C07K 7/08
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
7Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
04Linear peptides containing only normal peptide links
08having 12 to 20 amino acids
Applicants
  • 山东大学 SHANDONG UNIVERSITY [CN]/[CN]
Inventors
  • 吉爱国 JI, Aiguo
  • 宋淑亮 SONG, Shuliang
Agents
  • 济南圣达知识产权代理有限公司 JINAN SHENGDA INTELLECTUAL PROPERTY AGENCY CO., LTD.
Priority Data
201910394108.613.05.2019CN
Publication Language Chinese (ZH)
Filing Language Chinese (ZH)
Designated States
Title
(EN) HIRUDIN AND APPLICATION THEREOF
(FR) HIRUDINE ET APPLICATION ASSOCIÉE
(ZH) 一种水蛭肽及其应用
Abstract
(EN)
Provided in the present invention are hirudin and an application thereof. Specifically disclosed is synthetic hirudin provided with the activity of inhibiting the migration of macrophages in vitro, which may be applied to the prevention and development of atherosclerosis. The present invention firstly prepares a hirudin hydrolysate HE from Whitmania pigra. By means of further analysis and sequencing, the amino acid sequence of hirudin HE4-1 is finally obtained and artificially synthesized. By means of tests, it is verified that the artificially synthesized hirudin also has the activity of inhibiting the migration of macrophages, and thus has good, practical application value.
(FR)
La présente invention concerne l'hirudine et une application de cette dernière. Elle porte plus précisément sur une hirudine synthétique pourvue de l'activité consistant à inhiber la migration de macrophages in vitro, et qui peut être appliquée à la prévention et au développement de l'athérosclérose. La présente invention prépare d'abord un hydrolysat d'hirudine HE à partir de Whitmania pigra. Grâce à une analyse et un séquençage ultérieurs, la séquence d'acides aminés de l'hirudine HE4-1 est finalement obtenue et artificiellement synthétisée. Au moyen de tests, on vérifie que l'hirudine artificiellement synthétisée présente aussi l'activité d'inhibition de la migration des macrophages, et donc présente une bonne valeur pratique à l'application.
(ZH)
本发明提供一种水蛭肽及其应用,具体的,本发明公开了一种具有体外抑制巨噬细胞迁移活性的合成水蛭肽,其可应用于动脉粥样硬化的预防和发展。本发明首先从宽体金线蛭中制备得到水蛭酶解物HE,经进一步分析、测序,最终获得水蛭肽HE4-1的氨基酸序列并进行人工合成,经试验验证,人工合成的水蛭肽同样具有抑制巨噬细胞迁移活性,因此具有良好的实际应用之价值。
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